ORIGINAL ARTICLE
Green tea extract prevents the development of nonalcoholic liver steatosis in rats fed a high-fat diet
1
Electron Microscopy Laboratory, Chair and Department of Clinical Pathomorphology, Poznan University of Medical Sciences, Poland
2
Institute of Human Nutrition and Dietetics, Poznañ University of Life Sciences, Poland
3
Department of Pediatric Gastroenterology and Metabolic Diseases, Poznan University of Medical Sciences, Poland
4
Department of Oncologic Pathology and Prophylaxis, Poznan University of Medical Sciences, Greater Poland Cancer Center, Poznan, Poland
5
Chair and Department of Clinical Pathomorphology, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Poland
Submission date: 2018-11-27
Final revision date: 2019-02-22
Acceptance date: 2019-04-28
Publication date: 2020-03-06
Pol J Pathol 2019;70(4):295-303
KEYWORDS
TOPICS
ABSTRACT
Green tea contains many polyphenolic constitutes, which might prevent non-alcoholic fatty liver disease (NAFLD). We aimed to investigate whether green tea extract (GTE) given at doses reflecting habitual consumption of green tea beverages prevents development of NAFLD in rats fed a high-fat diet (HFD).
Twenty-four male Wistar rats were randomly divided into four equal groups (two study and two control groups). The study groups received a HFD (approximately 50% energy from fat), enriched with 1.1% and 2.0% GTE, respectively, for a total of 56 days. The control groups were fed a HFD alone and normal standardised diet (low-fat diet), respectively, for the same period of time.
The percentage of hepatocytes affected by steatosis in the HFD group (median [1st-3rd quartile]: 25% [12-34%]) was higher (p < 0.033 and p < 0.050, respectively) than in the HFD-2.0%GTE group (9% [3-18%]) and normal diet group (10% [5-18%]). No significant differences were observed for the group consuming HFD-1.1%GTE, in which intermediate results were observed (15% [4-30%]).
This finding points towards the hepatoprotective potential of GTE in preventing dietary-induced liver steatosis. In view of the increasing incidence of overweight and obesity a simple and cheap dietary modification, such as GTE supplementation, could prove to be useful clinically.
Twenty-four male Wistar rats were randomly divided into four equal groups (two study and two control groups). The study groups received a HFD (approximately 50% energy from fat), enriched with 1.1% and 2.0% GTE, respectively, for a total of 56 days. The control groups were fed a HFD alone and normal standardised diet (low-fat diet), respectively, for the same period of time.
The percentage of hepatocytes affected by steatosis in the HFD group (median [1st-3rd quartile]: 25% [12-34%]) was higher (p < 0.033 and p < 0.050, respectively) than in the HFD-2.0%GTE group (9% [3-18%]) and normal diet group (10% [5-18%]). No significant differences were observed for the group consuming HFD-1.1%GTE, in which intermediate results were observed (15% [4-30%]).
This finding points towards the hepatoprotective potential of GTE in preventing dietary-induced liver steatosis. In view of the increasing incidence of overweight and obesity a simple and cheap dietary modification, such as GTE supplementation, could prove to be useful clinically.
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