ORIGINAL ARTICLE
Candida Albicans Alcohol Dehydrogenase 1 gene in oral dysplasia and oral squamous cell carcinoma
1
Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Cairo University, Egypt
2
Department of Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Egypt
3
Department of Cancer Pathology, National Cancer Institute, Cairo University, Egypt
Submission date: 2018-02-26
Final revision date: 2018-06-07
Acceptance date: 2018-08-02
Publication date: 2019-12-08
Pol J Pathol 2019;70(3):210-216
KEYWORDS
TOPICS
ABSTRACT
This study was designed to examine the prevalence of Candida albicans alcohol dehydrogenase 1 (CaADH1) gene in oral dysplasia as well as oral squamous cell carcinoma (OSCC) with and without lymph node metastasis (LNM) using reverse transcription polymerase chain reaction (RT-PCR) in order to determine its role in initiation, propagation and metastasis of oral dysplasia and carcinoma. Formalin-fixed parafin-embedded specimens were grouped into four groups: 7 control, 16 oral dysplasia, 16 OSCC without LNM and 15 OSCC with LNM. All specimens were examined by periodic acid Schiff (PAS) stain to detect Candida hyphae, while CaADH1 gene was detected using RT-PCR. Candida hyphae were detected by PAS stain in one specimen of oral dysplasia group, 5 OSCC without LNM and 5 OSCC with LNM. CaADH1 gene was detected in 29 specimens (one case of sever dysplasia, 16 OSCC without LNM and 12 OSCC with LNM), with a highly statistically significant between the groups. CaADH1 gene is associated with OSCC with and without metastasis whereas in oral dysplasia it could not be estimated. Further studies with larger sample size are needed to confirm the role of CaADH1 in oral dysplasia and OSCC.
REFERENCES (19)
1.
El-Naggar A, Chan J, Grandis J, et al. WHO classification of head and neck tumours. El-Naggar A (4th ed.). IARC, Lyon 2017; 109-110.
2.
Jepsen A, Winther JE. Mycotic infection in oral leukoplakia. Acta Odontol Scand 1965; 23: 239-256.
3.
Sitheeque M, Samaranayake L. Chronic hyperplastic candidosis/candidiasis (candidal leukoplakia). Crit Rev Oral Biol Med 2003; 14: 253-267.
4.
Lucenteforte E, Garavello W, Bosetti C, et al. Dietary factors and oral and pharyngeal cancer risk. Oral Oncol 2009; 45: 461-467.
5.
Bakri M, Cannon R, Holmes A, et al. Detection of Candida albicans ADH1 and ADH2 mRNAs in human archival oral biopsy samples. J Oral Pathol Med 2014; 43: 704-710.
6.
Bakri M, Rich A, Cannon R, et al. In vitro expression of Candida albicans alcohol dehydrogenase genes involved in acetaldehyde metabolism. Mol Oral Microbiol 2015; 30: 27-38.
7.
Meurman J. Infectious and dietary risk factors of oral cancer. Oral Oncol 2010; 46: 411-413.
8.
Kang J, He Y, Hetzl D, et al. A Candida assessment of the link between oral candida containing biofilms and oral cancer. Adv Microbiol 2016; 6: 115-123.
9.
Bakri M, Hussaini H, Holmes A, et al. Revisiting the association between candidal infection and carcinoma, particularly oral squamous cell carcinoma. J Oral Microbial 2010; 2: 5780.
10.
Marttila E, Uittamo J, Rusanen P, et al. Acetaldehyde production and microbial colonization in oral squamous cell carcinoma and oral lichenoid disease. Oral Surg Oral Med Oral Pathol Oral Radiol 2013; 116: 61-68.
11.
Sanketh D, Patil S, Rao R. Estimating the frequency of Candida in oral squamous cell carcinoma using Calcofluor White fluorescent stain. J Investig Clin Dent 2016; 7: 304-307.
12.
Kumar RS, Ganvir S, Hazarey V. Candida and Calcofluor White: study in precancer and cancer. J Oral Maxillofac Pathol 2009; 13: 2-8.
13.
Jahanshahi G, Shirani S. Detection of Candida albicans in oral squamous cell carcinoma by fluorescence staining technique. Dent Res J 2015; 12: 115-120.
14.
Rodríguez-Cuesta J, Hernando F, Mendoza L, et al. Candida albicans enhances experimental hepatic melanoma metastasis. Clin Exp Metastasis 2010; 27: 35-42.
15.
Ramirez-Garcia A, Rementeria A, Aguirre-Urizar J, et al. Candida albicans and cancer: Can this yeast induce cancer development or progression? Crit Rev Microbiol 2014; 42: 181-193.
16.
Patil S, Rao R, Majumdar B, et al. Clinical appearance of oral candida infection and therapeutic strategies. Front Microbiol 2015; 6: 1391.
17.
Wu L, Feng J, Shi L, et al. Candidal infection in oral leukoplakia: a clinicopathologic study of 396 patients from eastern China. Ann Diagn Pathol 2013; 17: 37-40.
18.
Fox E, Nobile C. A sticky situation: untangling the transcriptional network controlling biofilm development in Candida albicans. Transcription 2012; 3: 315-322.
19.
Johnson N, Jayasekara P, Amarasinghe A. Squamous cell carcinoma and precursor lesions of the oral cavity: epidemiology and aetiology. Periodontol 2000 2011; 57: 19-37.
| eISSN: | 2084-9869 |
| ISSN: | 1233-9687 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
