ORIGINAL ARTICLE
Effect of lentinan on proliferation and apoptosis of human astrocytoma U251 cells
1
Department of Neurology, Jiangxi Provincial People’s Hospital Affiliated to Nanchang University, PR China
2
Department of Neurosurgery, Jiangxi Qianshan County People's Hospital, PR China
3
Department of Neurosurgery, Jiangxi Provincial People’s Hospital Affiliated to Nanchang University, PR China
Submission date: 2021-01-05
Final revision date: 2021-07-07
Acceptance date: 2021-08-17
Publication date: 2022-09-09
Pol J Pathol 2023;74(2):136-140
KEYWORDS
TOPICS
ABSTRACT
Aim of the study:
Aim of the study is To investigate the effect of lentinan on proliferation and apoptosis of human astrocytoma U251 cells. Lentinan was dissolved in DMEM complete medium to form different concentrations (0, 25, 50, 100, 200, 400, 500, 600 µg/ml). CCK8 was used to detect the effect of lentinan with different concentrations on proliferation of human astrocytoma U251 cells, and the expression of Ki-67 was detected by immunofluorescence. In addition, the effect of different concentrations of lentinan on apoptosis of human astrocytoma U251 cells was detected by flow cytometry. Compared with the blank control group, 50 and 100 µg/ml lentinan significantly promoted proliferation of human astrocytoma U251 cells. When the concentration is more than 100 µg/ml, the cell activity gradually decreases, and the cell activity is the lowest when the concentration is 600 µg/ml. In addition, the low concentration lentinan (25, 50, and 100 µg/ml) had no significant effect on apoptosis of human astrocytoma U251 cells. However, lentinan above 200 µg/ml significantly promoted apoptosis of human astrocytoma U251 cells and had a concentration gradient effect, and the highest apoptosis rate was at 600 µg/ml.
Conclusions:
Lentinan can effectively inhibit proliferation and promote apoptosis of human astrocytoma U251 cells.
Aim of the study is To investigate the effect of lentinan on proliferation and apoptosis of human astrocytoma U251 cells. Lentinan was dissolved in DMEM complete medium to form different concentrations (0, 25, 50, 100, 200, 400, 500, 600 µg/ml). CCK8 was used to detect the effect of lentinan with different concentrations on proliferation of human astrocytoma U251 cells, and the expression of Ki-67 was detected by immunofluorescence. In addition, the effect of different concentrations of lentinan on apoptosis of human astrocytoma U251 cells was detected by flow cytometry. Compared with the blank control group, 50 and 100 µg/ml lentinan significantly promoted proliferation of human astrocytoma U251 cells. When the concentration is more than 100 µg/ml, the cell activity gradually decreases, and the cell activity is the lowest when the concentration is 600 µg/ml. In addition, the low concentration lentinan (25, 50, and 100 µg/ml) had no significant effect on apoptosis of human astrocytoma U251 cells. However, lentinan above 200 µg/ml significantly promoted apoptosis of human astrocytoma U251 cells and had a concentration gradient effect, and the highest apoptosis rate was at 600 µg/ml.
Conclusions:
Lentinan can effectively inhibit proliferation and promote apoptosis of human astrocytoma U251 cells.
REFERENCES (20)
1.
Wu X, Li Y, Wan X, et al. Down-regulation of neogenin accelerated glioma progression through promoter methylation and its overexpression in SHG-44 induced apoptosis. PLOS One 2012; 7: e38074.
2.
Zhang CP, Li HQ, Zhang WT, et al. Clinical manifestations and imaging characteristics of gliomatosis cerebri with pathological confirmation. Asian Pac J Cancer Prev 2014; 15: 4487-4491.
3.
Scharl S, Straube C, Meyer B, et al. Critical consideration of the European Association for Neuro-Oncology (EANO) guideline on the diagnosis and treatment of adult astrocytic and oligodendroglial gliomas. Strahlenther Onkol 2017; 193: 984-988.
4.
van den Bent MJ, Chang SM. Grade II and III Oligodendroglioma and astrocytoma. Neurol Clin 2018; 36: 467-484.
5.
Chihara G, Maeda Y, Hamuro J, et al. Inhibition of mouse sarcoma 180 by polysaccharides from Lentinus edodes (Berk.) sing. Nature 1969; 222: 687-688.
6.
Wang Y, Jin H, Yu J, et al. Quality control and immunological activity of lentinan samples produced in China. Int J Biol Macromol 2020; 159: 129-136.
7.
Deng S, Zhang G, Kuai J, et al. Lentinan inhibits tumor angiogenesis via interferon γ and in a T cell independent manner. J Exp Clin Cancer Res 2018; 37: 260.
8.
Liu HJ, Qin Y, Zhao ZH, et al. Lentinan-functionalized Selenium nanoparticles target tumor cell mitochondria via TLR4/TRAF3/MFN1 pathway. Theranostics 2020; 10: 9083-9099.
9.
Wang XE, Wang YH, Zhou Q, et al. Immunomodulatory Effect of lentinan on aberrant T subsets and cytokines profile in non-small cell lung cancer patients. Pathol Oncol Res 2020; 26: 499-505.
10.
Liu Y, Zhao J, Zhao Y, et al. Therapeutic effects of lentinan on inflammatory bowel disease and colitis-associated cancer. J Cell Mol Med 2019; 23: 750-760.
11.
Zhang M, Zhang Y, Zhang L, Tian Q. Mushroom polysaccharide lentinan for treating different types of cancers: A review of 12 years clinical studies in China. Prog Mol Biol Transl Sci 2019; 163: 297-328.
12.
Yoshino S, Yoshida S, Maeda N, et al. [Clinical evaluation of the combination treatment of intrapleural or intraperitoneal administration of lentinan and OK-432 for malignant effusion]. Gan To Kagaku Ryoho 2010; 37: 2798-800.
13.
Li HL, Han PH, Pan DQ, et al. LncRNA XIST regulates cell proliferation, migration and invasion of glioblastoma via regulating miR-448 and ROCK1. J Biol Regul Homeost Agents 2020; 34: 2049-2058.
14.
Weller M, van den Bent M, Tonn JC, et al. European Association for Neuro-Oncology (EANO) Task Force on Gliomas. European Association for Neuro-Oncology (EANO) guideline on the diagnosis and treatment of adult astrocytic and oligodendroglial gliomas. Lancet Oncol 2017; 18: e315-e329.
15.
Congcong Q, Hengting Z, Shuhui L, et al. Evaluation of efficacy and safety for lentinan in the control of the malignant pleural effusions via intrapleural injection. Am J Med Sci 2019; 358: 400-411.
16.
Ina K, Furuta R, Kataoka T, et al. Chemo-Immunotherapy Using Lentinan for the Treatment of Gastric Cancer with Liver Metastases. Med Sci (Basel) 2016; 4: 8.
17.
Liu YR, Sun B, Zhu GH, et al. Selenium-lentinan inhibits tumor progression by regulating epithelial-mesenchymal transition. Toxicol Appl Pharmacol 2018; 360: 1-8.
18.
Souza PO, Bianchi SE, Figueiro F, et al. Anticancer activity of flavonoids isolated from Achyrocline satureioides in gliomas cell lines. Toxicol In Vitro 2018; 51: 23-33.
19.
Zhang Y, Hu Y, Li M, et al. The Traditional Chinese Medicine Compound, GRS, Alleviates Blood-Brain Barrier Dysfunction. Drug Des Devel Ther 2020; 14: 933-947.
20.
Zhang X, Liu T, Fan X, Ai N. In silico modeling on ADME properties of natural products: Classification models for blood-brain barrier permeability, its application to traditional Chinese medicine and in vitro experimental validation. J Mol Graph Model 2017; 75: 347-354.
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