ORIGINAL ARTICLE
Immunohistochemical investigation of endometrial leukocytes in implantation period in rats with streptozotosin-induced diabetes
Submission date: 2015-09-14
Acceptance date: 2016-03-13
Publication date: 2016-05-10
Pol J Pathol 2016;67(1):54-59
KEYWORDS
TOPICS
ABSTRACT
Our first aim was to determine the total leukocyte profile in implantation. Second aim was to detect the changes in uterine leukocyte profile in diabetes, a common accompanying disease. For this purpose 4 groups are formed with Wistar albino rats weighing 250-300 g. Two of the groups were non-diabetic and two of them were diabetic. One of the diabetic and one of the non-diabetic groups were left pregnant. Then uterus tissues of pregnant animals were removed in the 5th and 7th days of pregnancy together with tissues of other two non-pregnant groups. Tissues were analyzed immunohistochemically with antibodies CD45, CD3, CD4, CD8, CD56, CD68 and CD79a. It was revealed that pregnancy increased immune staining of CD68, CD3, CD45 and CD56 in endometrium. In addition it was observed that immune staining density of CD68, CD45 and CD56 decreased in diabetes. In the histopathological examination, significant degeneration was detected in the endometrium of diabetic rats. Diabetes could decrease leukocyte proportions in decidua in early pregnancy periods. Therefore immune cell therapies could be administrated in diabetes related problems of pregnancy.
REFERENCES (37)
1.
Bulla R, Fischetti F, Bossi F, et al. Feto-maternal immune interaction at the placental level. Lupus 2004; 13: 625-629.
2.
Bonatz G, Hansmann ML, Buchholz F, et al. Macrophage- and lymphocyte-subtypes in the endometrium during different phases of the ovarian cycle. Int J Gynaecol Obstet 1992; 37: 29-36.
3.
Song JY, Fraser IS. Effects of progestogens on human endometrium. Obstet Gynecol Surg 1995; 50: 385-394.
4.
Bulmer JN, Longfellow M, Ritson A. Leukocytes and resident blood cells in endometrium. Ann N Y Acad Sci 1991; 622: 57-68.
5.
Klentzeris LD, Bulmer JN, Warren A, et al. Endometrial lymphoid tissue in the timed endometrial biopsy: morphometric and immunohistochemical aspects. Am J Obstet Gynecol 1992; 167: 667-674.
6.
Loke YW, King A, Burrows TD. Decidua in human implantation. Hum Reprod 1995; 10: 14-21.
7.
Salamonsen LA, Woolley DE. Menstruation: induction by matrix metalloproteinases and inflammatory cells. J Reprod Immunol 1999; 44: 1-27.
8.
Trundley A, Moffett A. Human uterine leukocytes and pregnancy. Tissue Antigens 2004; 63: 1-12.
9.
Rieger L, Segerer S, Bernar T, et al. Specific subsets of immune cells in human decidua differ between normal pregnancy and preeclampsia – a prospective observational study. Reprod Biol Endocrinol 2009; 7: 132.
10.
Mor G, Straszewski-Chavez S, Abrahams V. Macrophage-trophoblast interactions. Methods Mol Med 2006; 122: 149-163.
11.
Bulmer JN, Lunny DP, Hagin SV. Immunohistochemical characterization of stromal leucocytes in nonpregnant human endometrium. Am J Reprod Immunol Microbiol 1988; 17: 83-90.
13.
Kimber SJ. Leukaemia inhibitory factor in implantation and uterine biology. Reproduction 2005; 130: 131-145.
14.
Koç A, Kanter M. SIçanlarda implantasyonda endometriyum dokusunun hücresel ve sIvIsal savunma sistemi hücreleri üzerinde histokimyasal ve histometrik araştIrmalar. Yüzüncü YIl Üniversitesi SağlIk Bilimleri Dergisi 2000; 6: 122-130.
15.
Starkey PM, Sargent IL, Redman CW. Cell populations in human early pregnancy decidua: characterization and isolation of large granular lymphocytes by flow cytometry. Immunology 1988; 65: 129-134.
16.
Santoni A, Carlino C, Gismondi A. Uterine NK cell development, migration and function. Reprod Biomed Online 2008; 16: 202-210.
17.
Kühnert M, Strohmeier R, Stegmuller M, et al. Changes in lymphocyte subsets during normal pregnancy. Eur J Obstet Gynecol Reprod Biol 1998; 76: 147-151.
18.
Mills JL, Simpson JL, Driscoll SG, et al. Incidence of spontaneous abortion among normal women and insulin-dependent diabetic women whose pregnancies were identified within 21 days of conception. N Engl J Med 1988; 319: 1617-1623.
19.
Davies JK, Shikes RH, Sze CI, et al. Histologic inflammation in the maternal and fetal compartments in a rabbit model of acute intra-amniotic infection. Am J Obstet Gynecol 2000; 183: 1088-1093.
20.
Mincheva-Nilsson L, Baranov V, Yeung MM, et al. Immunomorphologic studies of human decidua-associated lymphoid cells in normal early pregnancy. J Immunol 1994; 152: 2020-2032.
21.
Ho HN, Chao KH, Chen CK, et al. Activation status of T and NK cells in the endometrium throughout menstrual cycle and normal and abnormal early pregnancy. Hum Immunol 1996; 49: 130-136.
22.
Savion S, Gidon-Dabush S, Fein A, et al. Diabetes teratogenicity is accompanied by alterations in macrophages and T cell subpopulations in the uterus and lymphoid organs. Int Immunopharmacol 2004; 4: 1319-1327.
23.
Slukvin II, Breburda EE, Golos TG. Dynamic changes in primate endometrial leukocyte populations: differential distribution of macrophages and natural killer cells at the rhesus monkey implantation site and in early pregnancy. Placenta 2004; 25: 297-307.
24.
Shi Y, Ling B, Zhou Y, et al. Interferon-gamma expression in natural killer cells and natural killer T cells is suppressed in early pregnancy. Cell Mol Immunol 2007; 4: 389-394.
25.
Moffett-King A. Natural killer cells and pregnancy. Nat Rev Immunol 2002; 2: 656-663.
26.
Croy BA, van den Heuvel MJ, Borzychowski AM, et al. Uterine natural killer cells: a specialized differentiation regulated by ovarian hormones. Immunol Rev 2006; 214: 161-185.
27.
Kamat BR, Isaacson PG. The immunocytochemical distribution of leukocytic subpopulations in human endometrium. Am J Pathol 1987; 127: 66-73.
28.
Stewart JA, Bulmer JN, Murdoch AP. Endometrial leucocytes: expression of steroid hormone receptors. J Clin Pathol 1998; 51: 121-126.
29.
Quack KC, Vassiliadou N, Pudney J, et al. Leukocyte activation in the decidua of chromosomally normal and abnormal fetuses from women with recurrent abortion. Hum Reprod 2001; 16: 949-955.
30.
Ozenci CC, Korgun ET, Demir R. Immunohistochemical detection of CD45+, CD56+, and CD14+ cells in human decidua during early pregnancy. Early Pregnancy 2001; 5: 164-175.
31.
Starkey PM, Clover LM, Rees MC. Variation during the menstrual cycle of immune cell populations in human endometrium. Eur J Obstet Gynecol Reprod Biol 1991; 39: 203-207.
32.
Vassiliadou N, Bulmer JN. Quantitative analysis of T lymphocyte subsets in pregnant and non-pregnant human endometrium. Biol Reprod 1996; 55: 1017-1022.
33.
Kim JS, Romero R, Cushenberry E, et al. Distribution of CD14+ and CD68+ macrophages in the placental bed and basal plate of women with preeclampsia and preterm labor. Placenta 2007; 28: 571-576.
34.
Garris DR, Garris BL. Diabetes-induced, progressive endometrial involution characterization of periluminal epithelial lipoatrophy. Diabetes 2003; 52: 51-58.
35.
Garris DR, Williams S, Smith-West C, et al. Diabetes-associated endometrial disruption in the Chinese hamster: structural changes in relation to progressive hyperglycemia. Gynecol Obstet Invest 1984; 17: 293-300.
36.
Garris DR, Garris BL. Lipoatrophic diabetes-associated utero-ovarian dysfunction: influence of cellular lipid deposition on norepinephrine indices. Horm Res 2002; 58: 120-127.
37.
Garris DR, West RL, Pekala PH. Ultrastructural and metabolic changes associated with reproductive tract atrophy and adiposity in diabetic female mice. Anat Rec 1986; 216: 359-366.
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