ORIGINAL ARTICLE
Mast cells influence neoangiogenesis in prostatic cancer independently of ERG status
1
Department of Pathomorphology, Collegium Medicum of the Jagiellonian University, Krakow, Poland
2
Department of Urology, Collegium Medicum of the Jagiellonian University, Krakow, Poland
Submission date: 2016-05-16
Acceptance date: 2016-05-18
Publication date: 2016-11-25
Corresponding author
Krzysztof Okoń
Department of Pathomorphology Collegium Medicum of the Jagiellonian University Grzegórzecka 16 31-531 Krakow, Poland
Department of Pathomorphology Collegium Medicum of the Jagiellonian University Grzegórzecka 16 31-531 Krakow, Poland
Pol J Pathol 2016;67(3):244-249
KEYWORDS
TOPICS
ABSTRACT
A significant proportion of prostatic adenocarcinomas show recurrent translocation leading to ERG expression. Previously we found that ERG+ cases have higher microvessel density than negative ones. One factor influencing angiogenesis in cancer is mast cells. The aim of the present study was to evaluate the relationship between microvessels, mast cells and ERG status.
Tissue microarrays prepared from 113 radical prostatectomy specimens were analyzed with immunohistochemistry for CD31, tryptase and chymase. Vascular profiles and tryptase-positive and chymase-positive cells were counted.
The average number of tryptase-positive cells was 28.93/mm2 and chymase-positive cells 9.91/mm2. The average number of CD31+ vascular profiles was 352.66/mm2. The average number of tryptase-positive cells was 26.35/mm2 for ERG– cases and 32.12/mm2 for ERG+ cases. The average number of chymase-positive cells was 8.14/mm2 for ERG– cases and 12.06/mm2 for ERG+ cases. The average number of CD31+ vascular profiles was 321.34/mm2 for ERG– cases and 390.74/mm2 for ERG+ cases. The number of CD31+ vascular profiles was positively correlated with the number of tryptase-positive and chymase-positive cells (R = 0.26 and R = 0.20).
In summary, we demonstrated an interrelationship between mast cells, microvascular density and ERG status in prostatic carcinoma.
Tissue microarrays prepared from 113 radical prostatectomy specimens were analyzed with immunohistochemistry for CD31, tryptase and chymase. Vascular profiles and tryptase-positive and chymase-positive cells were counted.
The average number of tryptase-positive cells was 28.93/mm2 and chymase-positive cells 9.91/mm2. The average number of CD31+ vascular profiles was 352.66/mm2. The average number of tryptase-positive cells was 26.35/mm2 for ERG– cases and 32.12/mm2 for ERG+ cases. The average number of chymase-positive cells was 8.14/mm2 for ERG– cases and 12.06/mm2 for ERG+ cases. The average number of CD31+ vascular profiles was 321.34/mm2 for ERG– cases and 390.74/mm2 for ERG+ cases. The number of CD31+ vascular profiles was positively correlated with the number of tryptase-positive and chymase-positive cells (R = 0.26 and R = 0.20).
In summary, we demonstrated an interrelationship between mast cells, microvascular density and ERG status in prostatic carcinoma.
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