ORIGINAL ARTICLE
The potential beneficial effects of ethyl pyruvate on diabetic nephropathy: an experimental and ultrastructural study
1
Division of Transplantation, Department of General Surgery, Faculty of Medicine, University of Dicle, Diyabakir, Turkey
2
Division of Nephrology, Department of Internal Diseases, Faculty of Medicine, University of Dicle, Diyabakir, Turkey
3
Department of Histology and Embryology, Faculty of Medicine, University of Dicle, Diyabakir, Turkey
4
Department of Histology and Embryology, Faculty of Medicine, University of Çukurova, Adana, Turkey
5
Department of Pharmacology, Faculty of Medicine, University of Dicle, Diyabakir, Turkey
Submission date: 2016-01-19
Final revision date: 2016-04-13
Acceptance date: 2016-04-14
Publication date: 2016-11-25
Corresponding author
Mehmet Veysi Bahadir
Division of Transplantation Department of General Surgery Faculty of Medicine University of Dicle 21280 Diyarbakir, Turkey
Division of Transplantation Department of General Surgery Faculty of Medicine University of Dicle 21280 Diyarbakir, Turkey
Pol J Pathol 2016;67(3):250-257
KEYWORDS
TOPICS
ABSTRACT
Oxidative stress is one of the main causes of diabetic nephropathy, which is a complication of diabetes mellitus (DM). The aim of this study was to investigate the possible role of ethyl pyruvate (EP) in streptozotocin-induced diabetic rats’ kidney.
Four groups (n = 8) of male Wistar albino rats were used as follows: control group rats received only sodium citrate buffer solution intraperitoneally (ip). The EP group was given 50 mg/kg EP ip. In the DM group, diabetes was induced by streptozotocin. The DM + EP group received 50 mg/kg EP ip. All animals received daily treatment for 14 days, and at the end of the study the kidneys were removed: the left kidney of the rats was used for malondialdehyde (MDA) analysis and the right kidney for histological examination.
There was normal appearance of the kidney tissues in the control and the EP-administered groups. In the DM group, there was evident basement membrane thickening and enlargement of mesangial matrix; swelling in some tubular epithelial cells was also noticeable. In the DM+EP administered group, nearly the same appearance as the control group and relative thickening in the glomerular basal membrane were observed.
The antioxidant effect of ethyl pyruvate improved the renal structures in the DM + EP group.
Four groups (n = 8) of male Wistar albino rats were used as follows: control group rats received only sodium citrate buffer solution intraperitoneally (ip). The EP group was given 50 mg/kg EP ip. In the DM group, diabetes was induced by streptozotocin. The DM + EP group received 50 mg/kg EP ip. All animals received daily treatment for 14 days, and at the end of the study the kidneys were removed: the left kidney of the rats was used for malondialdehyde (MDA) analysis and the right kidney for histological examination.
There was normal appearance of the kidney tissues in the control and the EP-administered groups. In the DM group, there was evident basement membrane thickening and enlargement of mesangial matrix; swelling in some tubular epithelial cells was also noticeable. In the DM+EP administered group, nearly the same appearance as the control group and relative thickening in the glomerular basal membrane were observed.
The antioxidant effect of ethyl pyruvate improved the renal structures in the DM + EP group.
REFERENCES (39)
1.
Ahmet N. Advanced glycation end-products role in pathology of diabetic complications. Diabetes Res Clin Pract 2005; 67: 3-21.
2.
Forbes JM, Fukami K, Cooper ME. Diabetic nephropathy: where hemodynamics meets metabolism. Exp Clin Endocrinol Diabetes 2007; 115: 69-84.
3.
Schrijvers BF, De Vriese AS, Flyvbjerg A. From hyperglycemia to diabetic kidney disease: the role of metabolic, hemodynamic, intracellular factors and growth factors/cytokines. Endocr Rev 2004; 25: 971-1010.
4.
Ogawa D, Makino H. Pathogenesis of diabetic nephropathy: the role of inflammation. Nihon Rinsho 2012; 70 Suppl. 5: 389-392.
5.
Ramasamy R, Yan SF, Schmidt AM. Receptor for AGE (RAGE): signaling mechanisms in the pathogenesis of diabetes and its complications. Ann N Y Acad Sci 2011; 1243: 88-102.
6.
Sourris KC, Forbes JM. Interactions between advanced glycation end-products (AGE) and their receptors in the development and progression of diabetic nephropathy: are these receptors valid therapeutic targets. Curr Drug Targets 2009; 10: 42-50.
7.
Chung AC, Zhang H, Kong YZ, et al. Advanced glycation end-products induce tubular CTGF via TGF--independent Smad3 signaling. J Am Soc Nephrol 2010; 21: 249-260.
8.
Yamamoto Y, Gaynor RB. Role of the NF-kappa B pathway in the pathogenesis of human disease states. Curr Mol Med 2001; 1: 287-296.
9.
Kang KS, Kim HY, Yamabe N, Nagai R, Yokozawa T. Protective effect of sun ginseng against diabetic renal damage. Biol Pharm Bull 2006; 29: 1678-1684.
10.
Sappington PL, Han X, Yang R, et al. Ethyl pyruvate ameliorates intestinal epithelial barrier dysfunction in endotoxemic mice and immunostimulated caco-2 enterocytic monolayers. J Pharmacol Exp Ther 2003; 304: 464-476.
11.
Reade MC, Fink MP. Bench-to-bedside review: Amelioration of acute renal impairment using ethyl pyruvate. Crit Care 2005; 9: 556-560.
12.
Aras B, Tugcu V, Eren G, et al. Protective effect of pyrrolidine dithiocarbamate on kidney tissue in streptozotocin-induced diabetic rats. Turkish J Urol 2010; 36: 167-175.
13.
Tugcu V, Bas M, Ozbek E, et al. Pyrolidium dithiocarbamate prevents shockwave lithotripsy-induced renal injury through inhibition of nuclear factor-kappa B and inducible nitric oxide synthase activity in rats. J Endourol 2008; 22: 559-566.
14.
Payabvash S, Kiumehr S, Tavangar SM, Dehpour AR. Ethyl pyruvate reduces germ cell-specific apoptosis and oxidative stress in rat model of testicular torsion/detorsion. J Pediatr Surg 2008; 43: 705-712.
15.
Wang LW, Wang LK, Chen H. Ethyl pyruvate protects against experimental acute-on-chronic liver failure in rats. World J Gastroenterol 2012; 18: 5709-5718.
16.
Zhang Y, Li M, Meng M, Qin C. Effect of ethyl pyruvate on physical and immunological barriers of the small intestine in a rat model of sepsis. J Trauma 2009; 66: 1355-1364.
17.
Harada T, Moore BA, Yang R, et al. Ethyl pyruvate ameliorates ileus induced by bowel manipulation in mice. Surgery 2005; 138: 530-537.
18.
Cheng BQ, Liu CT, Li WJ, et al. Ethyl pyruvate improves survival and ameliorates distant organ injury in rats with severe acute pancreatitis. Pancreas 2007; 35: 256-261.
19.
Unal B, Karabeyoglu M, Huner T, et al. Ethyl pyruvate protects colonic anastomosis from ischemia-reperfusion injury. Surg Innov 2009; 16: 21-25.
20.
Tsung A, Kaizu T, Nakao A, et al. Ethyl pyruvate ameliorates liver ischemia-reperfusion injury by decreasing hepatic necrosis and apoptosis. Transplantation 2005; 79: 196-204.
21.
Akkoc H, Tunik S, Kelle I, et al. Ethyl pyruvate prevents ischemia reperfusion injury in isolated perfused rat heart. Lat Am J Pharm 2013; 32: 364-370.
22.
Akkoc H, Kelle I, Tunik S, et al. Effects of ethyl pyruvate on testicular damage in rats with streptozotocin-induced diabetes. Acta Endocrinologica 2012; 7: 35-45.
23.
Akkoc H, Kelle I, Tunik S, et al. Protective effect of ethyl pyruvate on liver injury in streptozotocin-induced diabetic rats. Acta Gastroenterol Belg 2012; 75: 336-341.
24.
Armstrong D, Al-Awadi L. Lipid peroxidation and retinopathy in streptozotocin-induced diabetes. Free Radic Biol Med 1991; 11: 433-436.
25.
Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol 1978; 52: 302-310.
26.
Noh H, Ha H, Yu MR, et al. High glucose increases inducible NO production in cultured rat mesangial cells. Possible role in fibronectin production. Nephron 2002; 90: 78-85.
27.
Kanter M. Protective effects of thymoquinone on streptozotocin-induced diabetic nephropathy. J Mol Hist 2009; 40: 107-115.
28.
Diabetes complications and control trial (DCCT) research group. The effect of diabetes on the development and progression of long-term complications of insulin dependent diabetes mellitus. N Engl J Med 1993; 329: 977-986.
29.
Lerco MM, Macedo CS, Silva RJ, Pinheiro Dde O, Spadella CT. The number of podocyte and slit diaphragm is decreased in experimental diabetic nephropathy. Acta Cir Bras 2006; 21: 87-91.
30.
Flyvbjerg A. Putative pathophysiological growth factor and cytokines in experimental diabetic kidney disease. Diabetologia 2000; 43: 1205-1223.
31.
Parving HH. Blockade of the renin-angiotensin-aldosterone system and renal protection in diabetes mellitus. J Renin Angiotensin Aldosterone Syst 2000; 1: 30-31.
32.
Erensoy N, Yilmazer S, Ozturk M, et al. Effects of ACE inhibition on the expression of type IV collagen and laminin in renal glomeruli in experimental diabetes. Acta Histochem 2004; 106: 279-287.
33.
Symons MCR, Gutteridge JMC. Free radicals and iron: Chemistry. Biology and Medicine. In: Applied Organometallic Chemistry. Oxford Uni Press, Oxford 1998; 283-284.
34.
Halliwell B. Free radicals antioxidants and human disease curiosity cause or consequence. Lancet 1994; 344: 721-724.
35.
West IC. Radicals and oxidative stress in diabetes. Diabet Med 2000; 17: 171-180.
36.
Halliwell B, Chirico S. Lipid peroxidation its mechanism measurement and significance. Am J Clin Nutr 1993; 57: 715S-725S.
37.
Rice Evans CA, Burdon RH. Free radical damage and its control. Formation of free radicals and mechanisms of action in norm biochemical processes and pathological states. Elsevier Science BV 1994; 1-393.
38.
Gutteridge JM. Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem 1995; 41: 1819-1828.
39.
Okutan H, Ozcelik N, Yilmaz HR, Uz E. Effects of caffeic acid phenethyl ester on lipid peroxidation and antioxidant enzymes in diabetic rat heart. Clin Biochem 2005; 38: 191-196.
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