ORIGINAL ARTICLE
Roles of CD3, CD4 and CD8 in synovial lymphocytes of rheumatoid arthritis
1
Department of Pathology, Tianjin Hospital, Tianjin, China
2
Department of Pathology, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China
Submission date: 2021-10-22
Final revision date: 2022-03-02
Acceptance date: 2022-03-14
Publication date: 2022-06-28
Pol J Pathol 2022;73(1):21-26
KEYWORDS
TOPICS
ABSTRACT
In this study, the immunohistochemical EnVision method was applied to detect CD3, CD4 and CD8 in synovial tissues of 40 patients with rheumatoid arthritis (RA) and 10 patients with osteoarthritis (OA). In 92.5% (37/40) RA cases, lymphocytes were focally aggregated, and even germinal centers appeared, forming lymphoid follicle-like structures. The expression of CD3, CD4, and CD8 were high in synovial tissue of RA group, but low in OA group. The number of CD3, CD4+, and CD8+ lymphocytes in OA group were significantly lower than that in RA group (p < 0.05); CD4+lymphocytes in RA accounted for the majority, and mostly were focally distributed. The number of CD8+lymphocytes in the synovial tissue were small, and were mostly scattered. The number of CD4+lymphocytes were significantly higher than CD8+lymphocytes (p<0.05). Compared with the OA group, the number of CD4+T and CD8+T lymphocytes in RA group were higher, and the ratio of CD4/CD8 was higher in RA group (p < 0.05). In conclusion, the CD3, CD4 and CD8 with high level may promote the occurrence and development of RA. The ratio of CD4+/CD8+ may be used as a reference index for the diagnosis and prognosis of RA.
REFERENCES (38)
1.
Zeng L, Li C, Jiang H, et al. Total saponins from Nigella glandulifera seeds ameliorate adjuvant-induced rheumatoid arthritis in rats by inhibition of an inflammatory response and bone erosion. Biomed Res Int 2021; 2021: 6613527.
2.
Luo XB, Xi JC, Liu Z, et al. Proinflammatory effects of ubiquitin-specific protease 5 (USP5) in rheumatoid arthritis fibroblast-like synoviocytes. Mediators Inflamm 2020; 2020: 8295149.
3.
Wright HL, Lyon M, Chapman EA, et al. Rheumatoid Arthritis synovial fluid neutrophils drive inflammation through production of chemokines, reactive oxygen species, and neutrophil extracellular traps. Front Immunol 2021; 11: 584116.
4.
Nogueira E, Gomes A, Preto A, Cavaco-Paulo A. Update on Therapeutic Approaches for Rheumatoid Arthritis. Curr Med Chem 2016; 23: 2190-203.
5.
Ma D, Xu K, Zhang G, et al. Immunomodulatory effect of human umbilical cord mesenchymal stem cells on T lymphocytes in rheumatoid arthritis. Int Immunopharmacol 2019; 74: 105687.
6.
Cho SH, Raybuck AL, Blagih J, et al. Hypoxia-inducible factors in CD4+ T cells promote metabolism, switch cytokine secretion, and T cell help in humoral immunity. Proc Natl Acad Sci U S A 2019; 116: 8975-8984.
7.
Moriguchi S. The role of vitamin E in T-cell differentiation and the decrease of cellular immunity with aging. Biofactors 2010, 7: 77-86.
8.
Kalia A, Agrawal M, Gupta N. CD8+ T cells are crucial for humoral immunity establishment by SA14-14-2 live attenuated Japanese encephalitis vaccine in mice. Eur J Immunol 2021; 51: 368-379.
9.
Appay V, Douek DC, Price DA. CD8+ T cell efficacy in vaccination and disease. Nat Med 2008; 14: 623-628.
10.
Guderud K, Sunde LH, Flåm ST, et al. Rheumatoid arthritis patients, both newly diagnosed and methotrexate treated, show more DNA methylation differences in CD4+ memory than in CD4+ naïve T cells. Front Immunol 2020; 11: 194.
11.
Wasén C, Ospelt C, Camponeschi A, et al. Nicotine changes the microRNA profile to regulate the FOXO memory program of CD8+ T cells in rheumatoid arthritis. Front Immunol 2020; 11: 1474.
12.
Yin X, Liu B, Wei H, et al. Activation of the Notch signaling pathway disturbs the CD4+/CD8+, Th17/Treg balance in rats with experimental autoimmune uveitis. Inflamm Res 2019; 68: 761-774.
13.
Kuwahara N, Kodaka T, Zushi Y, et al. T-cell large granular lymphocytic (LGL) leukemia consists of CD4+/CD8dim and CD4-/CD8+ LGL populations in association with immune thrombocytopenia, autoimmune neutropenia, and monoclonal B-cell lymphocytosis. J Clin Exp Hematop 2019; 59: 202-206.
14.
Nishigori R, Warabi Y, Shishido-Hara Y, et al. Inflammatory Cerebellar PML with a CD4/CD8 Ratio of 2.9 Showed a Favorable Prognosis in a Patient with Rheumatoid Arthritis. Intern Med 2019; 58: 3323-3329.
15.
Li EK, Tam LS, Wong CK, et al. Safety and efficacy of Ganoderma lucidum (lingzhi) and San Miao San supplementation in patients with rheumatoid arthritis: a double-blind, randomized, placebo-controlled pilot trial. Arthritis Rheum 2010; 57: 1143-1150.
16.
Pedersen JK, Kjaer NK, Svendsen AJ, Hørslev-Petersen K. Incidence of rheumatoid arthritis from 1995 to 2001: impact of ascertainment from multiple sources. Rheumatol Int 2009; 29: 411-415.
17.
Holoshitz J, Koning F, Coligan JE, et al. Isolation of CD4-CD8-mycobacteria-reactive T lymphocyte clones from rheumatoid arthritis synovial fluid. Nature 1989; 339: 226-229.
18.
Leah E. Rheumatoid arthritis: Metabolic reprogramming of T cells in RA. Nat Rev Rheumatol 2013; 9: 635-635.
19.
Mu L, Sun B, Kong Q, et al. Disequilibrium of T helper type 1, 2 and 17 cells and regulatory T cells during the development of experimental autoimmune myasthenia gravis. Immunol 2009, 128(1 Suppl): e826-e836.
20.
Hussein MR, Fathi NA, El-Din AME, et al. Alterations of the CD4(+), CD8 (+) T cell subsets, interleukins-1beta, IL-10, IL-17, tumor necrosis factor-alpha and soluble intercellular adhesion molecule-1 in rheumatoid arthritis and osteoarthritis: preliminary observations. Pathol Oncol Res 2008; 14: 321-328.
21.
Auger I, Roudier J. Influence of the QKRAA/QRRAA/RRRAA motifs of the third hypervariable region of HLA-DRB1 in the development of rheumatoid arthritis. J Rheumatol 1997; 24: 227-228.
22.
Castro F. Tumour necrosis factor microsatellites and HLA-DRB1*, HLA-DQA1*, and HLA-DQB1* alleles in Peruvian patients with rheumatoid arthritis. Ann Rheum Dis 2001; 60: 791-795.
23.
Sottini A, Imberti L, Gorla R, et al. Restricted expression of T cell receptor V but not V genes in rheumatoid arthritis. Eur J Immunol 2010; 21: 461-466.
24.
Scoazec JY, Feldmann G. Both macrophages and endothelial cells of the human hepatic sinusoid express the CD4 molecule, a receptor for the human immunodeficiency virus. Hepatology 2010, 12(3).
25.
Davies SP, Reynolds GM, Wilkinson AL, et al. Hepatocytes Delete Regulatory T Cells by Enclysis, a CD4+ T Cell Engulfment Process. Cell Rep 2019; 29: 1610-1620.e1614.
26.
Wang CR, Liu MF. Regulation of CCR5 expression and MIP production in CD4+ T cells from patients with rheumatoid arthritis. Clin Exp Immunol 2010; 132: 371-378.
27.
Wang BH, Fujisawa H, Zhuang LH, et al. CD4+ Th1 and CD8+ type 1 cytotoxic t cells both play a crucial role in the full development of contact hypersensitivity. J Immunol 2001; 165: 6783-6790.
28.
Chemin K, Gerstner C, Malmström V. Effector functions of CD4+ T cells at the site of local autoimmune inflammation-lessons from rheumatoid arthritis. Front Immunol 2019; 10: 353. .
29.
Jin-Sil P, Na-Rae K, Mi-Ae L, et al. Deficiency of IL-1 receptor antagonist suppresses IL-10-producing B cells in autoimmune arthritis in an IL-17/Th17-dependent manner. Immunol Lett 2018; 199: 44-52.
30.
Pawłowska J, Smoleńska, Daca A, et al. Older age of rheumatoid arthritis onset is associated with higher activation status of peripheral blood CD4+ T cells and disease activity. Clin Exp Immunol 2011; 163:157-164.
31.
Poole BCWR. The induction of arthritis in mice by the cartilage proteoglycan aggrecan: Roles of CD4+ and CD8+ T cells. Cell Immunol 1992; 144: 347-357.
32.
Dejaco C, Duftner C, Schirmer KM. Altered T-cell subtypes in spondyloarthritis, rheumatoid arthritis and polymyalgia rheumatica. Rheumatol Int 2010; 30: 297-303.
33.
Suzuki M, Konya C, Goronzy JJ, Weyand CM. Inhibitory CD8+ T cells in autoimmune disease. Hum Immunol 2008; 69: 781-789.
34.
Tada Y, Ho A, Koh DR, Mak TW. Collagen-induced arthritis in CD4- or CD8-deficient mice: CD8+ T cells play a role in initiation and regulate recovery phase of collagen-induced arthritis. J Immunol 1996; 156: 4520-4526.
35.
Criado G, Simelyte E, Inglis JJ, et al. Indoleamine 2,3 dioxygenase-mediated tryptophan catabolism regulates accumulation of Th1/Th17 cells in the joint in collagen-induced arthritis. Arthritis Rheum 2014; 60: 1342-1351.
36.
Weyand CM, Wu B, Goronzy JJ. The metabolic signature of T cells in rheumatoid arthritis. Curr Opin Rheumatol 2020; 32: 159-167.
37.
Appel H, Kuhne M, Spiekermann S, et al. Immunohistochemical analysis of hip arthritis in ankylosing spondylitis: evaluation of the bone-cartilage interface and subchondral bone marrow. Arthritis Rheum 2010; 54: 1805-1813.
38.
Nishigori R, Warabi Y, Shishido-Hara Y, et al. Inflammatory cerebellar PML with a CD4/CD8 ratio of 2.9 showed a favorable prognosis in a patient with rheumatoid arthritis: A Case Report. Intern Med 2019; 58: 3323-3329.
Share
RELATED ARTICLE
| eISSN: | 2084-9869 |
| ISSN: | 1233-9687 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
